LOCUS       Exported               12235 bp DNA     circular SYN 31-JUL-2025
DEFINITION  Gateway binary vector pGWB654 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12235)
  AUTHORS   Nakamura S, Mano S, Tanaka Y, Ohnishi M, Nakamori C, Araki M, Niwa 
            T, Nishimura M, Kaminaka H, Nakagawa T, Sato Y, Ishiguro S.
  TITLE     Gateway binary vectors with the bialaphos resistance gene, bar, as a
            selection marker for plant transformation
  JOURNAL   Biosci. Biotechnol. Biochem. 74 (6), 1315-1319 (2010)
  PUBMED    20530878
REFERENCE   2  (bases 1 to 12235)
  AUTHORS   Tanaka Y, Nakamura S, Ishiguro S, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12235)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12235)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 12235)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci.
            Biotechnol. Biochem."; date: "2010"; volume: "74"; issue: "6"; 
            pages: "1315-1319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1318..1333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1356..1386
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1440..2096
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2441..2743
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2787..2911)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2922..3596
                     /codon_start=1
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      3621..3873
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3907..3923)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3931..3947
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3955..3985)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4000..4021)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(4057..4309)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4472..5020)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(5072..5251)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5757..5781)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6302..7090
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7339..7927
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8113..8253)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8597..8791)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8860..9930)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10362..10988)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggcct
     1321 cgaggcctcg aggggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
     1381 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
     1441 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     1501 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     1561 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     1621 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     1681 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     1741 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     1801 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     1861 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     1921 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     1981 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     2041 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa
     2101 cgcgtggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt
     2161 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat
     2221 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat
     2281 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt
     2341 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
     2401 atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac
     2461 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     2521 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
     2581 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     2641 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     2701 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
     2761 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt
     2821 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     2881 tttctcgttc agctttcttg tacaaagtgg ttgataacag catggcctcc tccgaggacg
     2941 tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg ctccgtgaac ggccacgagt
     3001 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     3061 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtcccct cagttccagt
     3121 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     3181 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     3241 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     3301 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca
     3361 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagatg aggctgaagc
     3421 tgaaggacgg cggccactac gacgccgagg tcaagaccac ctacatggcc aagaagcccg
     3481 tgcagctgcc cggcgcctac aagaccgaca tcaagctgga catcacctcc cacaacgagg
     3541 actacaccat cgtggaacag tacgagcgcg ccgagggccg ccactccacc ggcgcctaag
     3601 cttagagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
     3661 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
     3721 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
     3781 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
     3841 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt ggttccggaa ccaattcgta
     3901 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     3961 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     4021 aggctgaatt aggcgcgcct atttctatta attcccgatc tagtaacata gatgacaccg
     4081 cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg tattaaatgt
     4141 ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg cattacatgt
     4201 taattattac atgcttaacg taattcaaca gaaattatat gataatcatc gcaagaccgg
     4261 caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatcg gggaaattcg
     4321 agctggggac gtccccagct cggtaccccc tcctgggcac ggtcgtacgc gatccgccgc
     4381 ggttccacct caccattgcg ggtatgccag ttgagttcga tgagttcccg taccagctcc
     4441 aactcgggcg atgccacgcg cactccgttc agatctcggt gacgggcagg accggacggg
     4501 gcggtaccgg caggctgaag tccagctgcc agaaacccac gtcatgccag ttcccgtgct
     4561 tgaagccggc cgcccgcagc atgccgcggg gggcatatcc gagcgcctcg tgcatgcgca
     4621 cgctcgggtc gttgggcagc ccgatgacag cgaccacgct cttgaagccc tgtgcctcca
     4681 gggacttcag caggtgggtg tagagcgtgg agcccagtcc cgtccgctgg tggcgggggg
     4741 agacgtacac ggtcgactcg gccgtccagt cgtaggcgtt gcgtgccttc caggggcccg
     4801 cgtaggcgat gccggcgacc tcgccgtcca cctcggcgac gagccaggga tagcgctccc
     4861 gcagacggac gaggtcgtcc gtccactcct gcggttcctg cggctcggta cggaagttga
     4921 ccgtgcttgt ctcgatgtag tggttgacga tggtgcagac cgccggcatg tccgcctcgg
     4981 tggcacggcg gatgtcggcc gggcgtcgtt ctgggctcat atcttattgc cccccgggat
     5041 cagatctgga ttgagagtga atatgagact ctaattggat accgagggga atttatggaa
     5101 cgtcagtgga gcatttttga caagaaatat ttgctagctg atagtgacct taggcgactt
     5161 ttgaacgcgc aataatggtt tctgacgtat gtgcttagct cattaaactc cagaaacccg
     5221 cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc
     5281 ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt ctccgctcat gatcttgatc
     5341 ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc
     5401 caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg
     5461 cccagtctag ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg
     5521 cgttttccct tgtccagata gcccagtagc tgacattcat ccggggtcag caccgtttct
     5581 gcggactggc tttctacgtg ttccgcttcc tttagcagcc cttgcgccct gagtgcttgc
     5641 ggcagcgtga agctgatccg tcgagattaa tagaaataaa ttcagcctaa ttcggcgtta
     5701 attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
     5761 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
     5821 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
     5881 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
     5941 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
     6001 gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg acataagcct gttcggttcg
     6061 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     6121 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
     6181 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     6241 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     6301 catgggggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     6361 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     6421 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     6481 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     6541 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     6601 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     6661 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     6721 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     6781 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     6841 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     6901 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     6961 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     7021 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     7081 agtcggcaaa taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc
     7141 ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa
     7201 gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc
     7261 atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7321 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7381 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7441 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     7501 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7561 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7621 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7681 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     7741 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     7801 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7861 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7921 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     7981 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8041 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     8101 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     8161 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     8221 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     8281 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     8341 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc
     8401 ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt
     8461 gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc
     8521 ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt
     8581 gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa
     8641 agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga
     8701 ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg
     8761 ctttgggttc ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc
     8821 tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc
     8881 ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt
     8941 caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt
     9001 cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc
     9061 tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat
     9121 caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg
     9181 gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac
     9241 gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt
     9301 ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac
     9361 caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac
     9421 gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga
     9481 ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc
     9541 ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc
     9601 agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc
     9661 gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg
     9721 cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg
     9781 atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg
     9841 ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg
     9901 taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg
     9961 ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca
    10021 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct
    10081 ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc
    10141 gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag
    10201 cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc
    10261 caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt
    10321 gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt
    10381 tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg
    10441 gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga
    10501 atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg
    10561 atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc
    10621 ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga
    10681 atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct
    10741 ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg
    10801 cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca
    10861 ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg
    10921 gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata
    10981 accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga
    11041 ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg
    11101 tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca
    11161 ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg
    11221 cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt
    11281 gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc
    11341 ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc
    11401 gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc
    11461 tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt
    11521 gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg
    11581 ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac
    11641 catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc
    11701 gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg
    11761 gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag
    11821 ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc
    11881 cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata
    11941 gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca
    12001 agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc
    12061 ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac
    12121 tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc
    12181 ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aacct
//
